Dynamic collision-induced dissociation (DCID) in a quadrupole ion trap using a two-frequency excitation waveform: I. Effects of excitation frequency and phase angle
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چکیده
منابع مشابه
Dynamic collision-induced dissociation (DCID) in a quadrupole ion trap using a two-frequency excitation waveform: I. Effects of excitation frequency and phase angle.
This study describes the application of a two-frequency excitation waveform to the end-cap electrodes of a quadrupole ion trap (QIT) during the mass acquisition period to deliberately fragment selected precursor ions. This approach obviates the need for a discrete excitation period and guarantees on-resonant excitation conditions without any requirement for resonant tuning; it is therefore fast...
متن کاملDynamic collision-induced dissociation (DCID) in a quadrupole ion trap using a two-frequency excitation waveform: II. Effects of frequency spacing and scan rate.
Dynamic CID of selected precursor ions is achieved by the application of a two-frequency excitation waveform to the end-cap electrodes during the mass instability scan of a quadrupole ion trap (QIT) mass spectrometer. This new method permits a shorter scanning time when compared with conventional on-resonance CID. When the excitation waveform consists of two closely-spaced frequencies, the rela...
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Fragmentation of the pentapeptide leucine enkephalin (YGGFL) is accomplished via higher-order resonances combined with simultaneous analysis of low-mass product ions. Two methods of achieving excitation are explored: (1) 0.5 ms resonant excitation at the omega and at Omega-omega secular frequencies of ion motion (where Omega is the radio-frequency (rf) drive frequency) in a manner similar to bo...
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The fragmentation of natural peptides using dynamic collision-induced dissociation (DCID), a novel fragmentation method for quadrupole ion traps, is demonstrated. Using leucine enkephalin as a diagnostic molecule, the fragmentation efficiencies and energetics of DCID are compared with other methods of collisional activation in ion traps such as conventional on-resonance excitation and high-ampl...
متن کاملMultigenerational Broadband Collision-Induced Dissociation of Precursor Ions in a Linear Quadrupole Ion Trap.
A method of fragmenting ions over a wide range of m/z values while balancing energy deposition into the precursor ion and available product ion mass range is demonstrated. In the method, which we refer to as "multigenerational collision-induced dissociation", the radiofrequency (rf) amplitude is first increased to bring the lowest m/z of the precursor ion of interest to just below the boundary ...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2007
ISSN: 1044-0305,1879-1123
DOI: 10.1016/j.jasms.2006.12.008